陶云龙博士，太阳成集团tyc33455cc特聘研究员，江苏特聘教授，博士生导师，国家级青年人才计划入选者。主要研究方向是以人多能干细胞的神经分化过程为模型，研究人类神经系统发育原理、神经系统疾病的发病机制和开发用于治疗神经系统疾病的细胞疗法。以第一作者或通讯作者身份在Nature Biotechnology (2024), Nature Medicine (2021), Cell Stem Cell (2016)，EMBO Reports (2020)等国际权威学术期刊发表文章。主持国家自然科学基金面上，江苏特聘教授等项目，参与国家重点研发专项（课题负责人）及国家重大专项项目（骨干）。

  教育背景

  工作经历

多能干细胞（包括胚胎干细胞和诱导多能干细胞）具备自我更新能力，并可分化为机体所有细胞类型。近年来，基因编辑、干细胞分化及单细胞测序等技术的飞速发展，使得多能干细胞被广泛应用于研究人类器官发育机制和疾病发生过程。相较于传统动物和细胞模型，人多能干细胞携带完整的人类基因组信息，能够再现人类特有的发育进程，在模拟人类器官尤其是神经系统的发育方面展现出独特优势。

陶云龙课题组以人多能干细胞为主要模型，致力于探究人类神经系统发育规律、神经系统疾病的致病机制及其细胞治疗策略。具体研究方向包括：

1．神经细胞亚型的精准分化

人类大脑包含丰富多样的神经细胞类型，它们在形态和功能上存在差异，并对疾病表现出不同的易感性。获得特定功能的神经细胞亚型，对于解析神经系统疾病的发生与发展至关重要。神经分化是从多能干细胞逐步产生形态与功能各异的神经细胞的过程，阐明其调控机制是理解神经系统发育以及实现特定神经亚型定向分化的基础。本课题组聚焦于深入探究特定神经细胞的发育轨迹，并实现其体外高效分化。

2．基于神经分化过程的分子机制与疾病关联研究

人多能干细胞经体外定向分化，可产生具有特定形态和功能的神经细胞亚型，这一动态过程与体内神经系统发育高度相似，为研究人类神经发育提供了理想模型。利用人多能干细胞携带人类完整遗传信息的优势，我们可探索人类特异的发育事件。本课题组综合运用多组学技术（如单细胞测序、RNA-Seq、ChIP-Seq等），系统解析神经细胞命运决定的分子机制，并探究特定神经亚型在神经系统疾病发生发展中的作用，为开发新型治疗策略奠定理论基础。

3．基于神经再生的细胞疗法开发

前期研究（包括本课题组及其他团队）表明，移植干细胞分化的神经细胞是治疗神经退行性疾病极具潜力的策略。因此，在建立高效神经分化体系的基础上，本课题组将积极推动细胞疗法在神经系统疾病治疗中的应用探索。

欢迎对干细胞、神经分化及神经再生研究感兴趣的同学加入本课题组。实验室常年招收本科生、研究生、博士后、科研助理及副研究员，有意者请与PI联系。

First (*) & Corresponding Author (#) Publications：

1. Yang Y, Tao Y#. Regenerating Locus Coeruleus-Norepinephrine (LC-NE) Function: A Novel Approach for Neurodegenerative Diseases. Cell Prolif. 2025 Jan 28;:e13807. doi: 10.1111/cpr.13807. PubMed PMID: 39876531.

2. Tao Y#, Li X, Dong Q, Kong L, Petersen AJ, Yan Y, Xu K, Zima S, Li Y, Schmidt DK, Ayala M, Mathivanan S, Sousa AMM, Chang Q, Zhang SC#. Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells. Nat Biotechnol. 2023 Nov 16;. doi: 10.1038/s41587-023-01977-4. PubMed PMID: 37974010.

3. Tao Y, Vermilyea SC, Zammit M, Lu J, Olsen M, Metzger JM, Yao L, Chen Y, Phillips S, Holden JE, Bondarenko V, Block WF, Barnhart TE, Schultz-Darken N, Brunner K, Simmons H, Christian BT, Emborg ME#, Zhang SC#. Autologous transplant therapy alleviates motor and depressive behaviors in parkinsonian monkeys. Nat Med. 2021 Apr;27(4):632-639. doi: 10.1038/s41591-021-01257-1. Epub 2021 Mar 1. PubMed PMID: 33649496; PubMed Central PMCID: PMC8198752.

4. Tao Y*, Cao J^*, Li M, Hoffmann B, Xu K, Chen J, Lu X, Guo F, Li X, Phillips MJ, Gamm DM, Chen H#, Zhang SC#. PAX6D instructs neural retinal specification from human embryonic stem cell-derived neuroectoderm. EMBO Rep. 2020 Sep 3;21(9):e50000. doi: 10.15252/embr.202050000. Epub 2020 Jul 23. PubMed PMID: 32700445; PubMed Central PMCID: PMC7507545.

5. Tao Y, Zhang SC#. Neural Subtype Specification from Human Pluripotent Stem Cells. Cell Stem Cell. 2016 Nov 3;19(5):573-586. doi: 10.1016/j.stem.2016.10.015. PubMed PMID: 27814479; PubMed Central PMCID: PMC5127287.

6. Huang CT^*, Tao Y*, Lu J, Jones JR, Fowler L, Weick JP, Zhang SC#. Time-Course Gene Expression Profiling Reveals a Novel Role of Non-Canonical WNT Signaling During Neural Induction. Sci Rep. 2016 Sep 7;6:32600. doi: 10.1038/srep32600. PubMed PMID: 27600186; PubMed Central PMCID: PMC5013468.

7. Tao Y, Wu Q, Guo X, Zhang Z, Shen Y, Wang F#. MBD5 regulates iron metabolism via methylation-independent genomic targeting of Fth1 through KAT2A in mice. Br J Haematol. 2014 Jul;166(2):279-91. doi: 10.1111/bjh.12863. Epub 2014 Apr 18. PubMed PMID: 24750026.

8. Tao Y, Wang Y, Rogers JT, Wang F#. Perturbed iron distribution in Alzheimer's disease serum, cerebrospinal fluid, and selected brain regions: a systematic review and meta-analysis. J Alzheimers Dis. 2014;42(2):679-90. doi: 10.3233/JAD-140396. Review. PubMed PMID: 24916541.

Co-Author Publications:

1. Yan Y, Li X, Gao Y, Mathivanan S, Kong L, Tao Y, Dong Y, Li X, Bhattacharyya A, Zhao X, Zhang SC. 3D bioprinting of human neural tissues with functional connectivity. Cell Stem Cell. 2024 Feb 1;31(2):260-274.e7. doi: 10.1016/j.stem.2023.12.009. PubMed PMID: 38306994; PubMed Central PMCID: PMC10883639.

2. Olsen ME, Brodsky EK, Oler JA, Riedel MK, Mueller SAL, Vermilyea SC, Metzger JM, Tao Y, Brunner KG, Ahmed AS, Zhang SC, Emborg ME, Kalin NH, Block WF. Real-time trajectory guide tracking for intraoperative MRI-guided neurosurgery. Magn Reson Med. 2023 Feb;89(2):710-720. doi: 10.1002/mrm.29426. Epub 2022 Sep 21. PubMed PMID: 36128887; PubMed Central PMCID: PMC9930741.

3. Giffin-Rao Y, Sheng J, Strand B, Xu K, Huang L, Medo M, Risgaard KA, Dantinne S, Mohan S, Keshan A, Daley RA Jr, Levesque B, Amundson L, Reese R, Sousa AMM, Tao Y, Wang D, Zhang SC, Bhattacharyya A. Altered patterning of trisomy 21 interneuron progenitors. Stem Cell Reports. 2022 Jun 14;17(6):1366-1379. doi: 10.1016/j.stemcr.2022.05.001. Epub 2022 May 26. PubMed PMID: 35623352; PubMed Central PMCID: PMC9214050.

4. Zammit M, Tao Y, Olsen ME, Metzger J, Vermilyea SC, Bjornson K, Slesarev M, Block WF, Fuchs K, Phillips S, Bondarenko V, Zhang SC, Emborg ME, Christian BT. [18F]FEPPA PET imaging for monitoring CD68-positive microglia/macrophage neuroinflammation in nonhuman primates. EJNMMI Res. 2020 Aug 6;10(1):93. doi: 10.1186/s13550-020-00683-5. PubMed PMID: 32761399; PubMed Central PMCID: PMC7410886.

5. Li X, Tao Y, Bradley R, Du Z, Tao Y, Kong L, Dong Y, Jones J, Yan Y, Harder CRK, Friedman LM, Bilal M, Hoffmann B, Zhang SC. Fast Generation of Functional Subtype Astrocytes from Human Pluripotent Stem Cells. Stem Cell Reports. 2018 Oct 9;11(4):998-1008. doi: 10.1016/j.stemcr.2018.08.019. Epub 2018 Sep 27. PubMed PMID: 30269954; PubMed Central PMCID: PMC6178885.

6. Wu Q, Shen Y, Tao Y, Wei J, Wang H, An P, Zhang Z, Gao H, Zhou T, Wang F, Min J. Hemojuvelin regulates the innate immune response to peritoneal bacterial infection in mice. Cell Discovery. 2017;3:17028. doi: 10.1038/celldisc.2017.28. eCollection 2017. PubMed PMID: 28815056; PubMed Central PMCID: PMC5556331.

7. Vermilyea SC, Lu J, Olsen M, Guthrie S, Tao Y, Fekete EM, Riedel MK, Brunner K, Boettcher C, Bondarenko V, Brodsky E, Block WF, Alexander A, Zhang SC, Emborg ME. Real-Time Intraoperative MRI Intracerebral Delivery of Induced Pluripotent Stem Cell-Derived Neurons. Cell Transplant. 2017 Apr 13;26(4):613-624. doi: 10.3727/096368916X692979. Epub 2016 Sep 14. PubMed PMID: 27633706; PubMed Central PMCID: PMC5539906.

8. Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, Zhang SC. Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Cell Stem Cell. 2015 Aug 6;17(2):233-44. doi: 10.1016/j.stem.2015.06.001. Epub 2015 Jul 2. PubMed PMID: 26145478; PubMed Central PMCID: PMC4530040.

9. Wu Q, Wang H, An P, Tao Y, Deng J, Zhang Z, Shen Y, Chen C, Min J, Wang F. HJV and HFE Play Distinct Roles in Regulating Hepcidin. Antioxid Redox Signal. 2015 May 20;22(15):1325-36. doi: 10.1089/ars.2013.5819. Epub 2015 Mar 19. PubMed PMID: 25608116; PubMed Central PMCID: PMC4410569.

10. Zhang Z, Guo X, Herrera C, Tao Y, Wu Q, Wu A, Wang H, Bartnikas TB, Wang F. Bmp6 expression can be regulated independently of liver iron in mice. PLoS One. 2014;9(1):e84906. doi: 10.1371/journal.pone.0084906. eCollection 2014. PubMed PMID: 24454764; PubMed Central PMCID: PMC3890292.

11. Guan Y, An P, Zhang Z, Zhang F, Yu Y, Wu Q, Shi Y, Guo X, Tao Y, Wang F. Screening identifies the Chinese medicinal plant Caulis Spatholobi as an effective HAMP expression inhibitor. J Nutr. 2013 Jul;143(7):1061-6. doi: 10.3945/jn.113.174201. Epub 2013 May 22. PubMed PMID: 23700338.

12. Guo X, Zhang Z, Zhang F, Tao Y, An P, Wu Q, Wang CY, Knutson MD, Wang F. Fine-mapping and genetic analysis of the loci affecting hepatic iron overload in mice. PLoS One. 2013;8(5):e63280. doi: 10.1371/journal.pone.0063280. Print 2013. PubMed PMID: 23675470; PubMed Central PMCID: PMC3651197.

13. Zhang F, Tao Y, Zhang Z, Guo X, An P, Shen Y, Wu Q, Yu Y, Wang F. Metalloreductase Steap3 coordinates the regulation of iron homeostasis and inflammatory responses. Haematologica. 2012 Dec;97(12):1826-35. doi: 10.3324/haematol.2012.063974. Epub 2012 Jun 11. PubMed PMID: 22689674; PubMed Central PMCID: PMC3590089.

14. Zhang Z, Zhang F, Guo X, An P, Tao Y, Wang F. Ferroportin1 in hepatocytes and macrophages is required for the efficient mobilization of body iron stores in mice. Hepatology. 2012 Sep;56(3):961-71. doi: 10.1002/hep.25746. Epub 2012 Aug 2. PubMed PMID: 22473803.

15. Zhang Z, Zhang F, An P, Guo X, Shen Y, Tao Y, Wu Q, Zhang Y, Yu Y, Ning B, Nie G, Knutson MD, Anderson GJ, Wang F. Ferroportin1 deficiency in mouse macrophages impairs iron homeostasis and inflammatory responses. Blood. 2011 Aug 18;118(7):1912-22. doi: 10.1182/blood-2011-01-330324. Epub 2011 Jun 24. PubMed PMID: 21705499.

所有文章列表:https://www.ncbi.nlm.nih.gov/myncbi/1Tu0585gZ9_5x/bibliography/public/

2022    优青（海外）

2023    江苏特聘教授

2024    国家自然科学基金面上项目（主持）

2024    国家重点研发计划青年科学家项目（课题负责人）

2025    国家科技重大专项青年科学家项目（骨干）